To establish stable knockdown of Wnt7a in 4T1 cells, cells were infected with Mission particles (Sigma) containing one of two independent Wnt7a shRNA (shWnt7a-88, TRCN0000071788 and shWnt7a-91, TRCN0000071791) or control shRNA (shCont; SHC001V) at an multiplicity of contamination of 2

To establish stable knockdown of Wnt7a in 4T1 cells, cells were infected with Mission particles (Sigma) containing one of two independent Wnt7a shRNA (shWnt7a-88, TRCN0000071788 and shWnt7a-91, TRCN0000071791) or control…