Free-floating sections were incubated with IBA-1, CD68 or GFAP antibody

Free-floating sections were incubated with IBA-1, CD68 or GFAP antibody. AI, agranular insular cortex; Pir, piriform cortex; AO, anterior olfactory bulb. Scale bar = 40 m.(TIF) pone.0159463.s002.tif (9.0M) GUID:?098FB7FC-A599-4DC3-9A04-E77370CA127B S3 Fig: Gliosis was observed in the amygdala in old EC-Tau mice. Free-floating sections were incubated with IBA-1, CD68 or GFAP antibody. IBA-1+ and CD68+ microglia and GFAP+ astrocytes were found to be recruited to the amygdala in mice with overt tau pathology, but not control mice. Scale bar = 100 m.(TIF) pone.0159463.s003.tif (9.2M) GUID:?F47FB5F8-7A6D-4698-BA60-7A5B5996458A S1 Movie: The movie of iDISCO+ immunolabeling of Alexa Fluor 647-CP27 in an 8-mo-old EC-Tau mouse. The movies were generated from 3D rendering as described in Materials and Methods. Different brain regions with tau pathology are indicated by superimposed artificial colors. The regions with no or very little tau immunoreactivity are not colored.(MP4) pone.0159463.s004.mp4 (21M) GUID:?4A76D689-B756-479C-920E-5D4AF817B9FC S2 Movie: The movie of iDISCO+ immunolabeling of Alexa Fluor 647-CP27 in a 14-mo-old EC-Tau mouse. Different brain regions with tau pathology are indicated by superimposed artificial colors. The regions with no or very little tau immunoreactivity are not colored.(MP4) pone.0159463.s005.mp4 (17M) GUID:?E6B248E2-FE55-488E-A23A-F4F571DFCC2F S3 Movie: The movie of iDISCO+ immunolabeling of Alexa Fluor 647-CP27 in a 25-mo-old EC-Tau mouse. Different brain regions with tau pathology are indicated by superimposed artificial colors. The regions with no or very little tau immunoreactivity are not colored.(MP4) pone.0159463.s006.mp4 (22M) GUID:?58618B4D-6A56-427A-AED4-4EA82E54BAD4 S4 Movie: The movie of iDISCO+ immunolabeling of Alexa Fluor 647-CP27 in a 34-mo-old EC-Tau mouse. Different brain regions with tau pathology are indicated by superimposed artificial colors. The regions with no or very little tau immunoreactivity are not colored.(MP4) pone.0159463.s007.mp4 (32M) GUID:?D9D9FE46-8F70-46B9-BEC0-14D466441A53 S5 Movie: The movie of iDISCO+ immunolabeling of Alexa Fluor 647-CP27 in a 16-mo-old uninduced Tau control mouse (Tg4510, no tTA). There was no positive tau immunoreactivity detected.(MP4) pone.0159463.s008.mp4 (1.4M) GUID:?A7CF3523-0E82-4B89-8E7D-BBBC0B804EAE S6 Movie: The movie of iDISCO+ immunolabeling of Alexa Fluor 647-CP27 in a 33-mo-old Neuropsin tTA (no 4510) control mouse. There was no positive tau immunoreactivity detected.(MP4) pone.0159463.s009.mp4 (1.6M) GUID:?F216A893-AF00-4C42-A7AA-D4E9E2FF3E77 Data Availability StatementAll relevant data are within the paper and its Supporting Information files. Abstract 3D volume imaging using iDISCO+ was applied to observe the spatial and temporal progression of tau pathology in deep structures of the brain of a mouse model that recapitulates the earliest stages of Alzheimers disease (AD). Tau pathology was compared at four timepoints, up to 34 months as it spread through the hippocampal formation and out into the neocortex along an anatomically connected route. Tau pathology was associated with significant gliosis. No evidence for uptake and accumulation of tau by glia was observed. Neuronal cells did appear to have internalized tau, including in extrahippocampal areas α-Estradiol as a small proportion of cells that had accumulated human tau protein did not express detectible levels of human tau mRNA. At α-Estradiol the oldest timepoint, mature tau pathology in the entorhinal cortex (EC) was associated with significant cell loss. As in human AD, mature tau pathology in the EC and the presence of tau pathology in the neocortex correlated with cognitive impairment. 3D Mouse monoclonal to LPL volume imaging is an ideal technique to easily monitor the spread of pathology over time in models of disease progression. Introduction A combination of brain clearing and immunolabeling has recently been used to visualize amyloid and tau lesions in 3D in blocks of postmortem tissue from late stage human AD brain [1C3]. α-Estradiol Additionally, amyloid deposits have been observed in intact mouse brain. However, the 3D visualization of tau pathology and the rigorous examination of how pathology distribution changes as the disease progresses has not been reported. Extracellular amyloid- (A) plaques and intraneuronal neurofibrillary tangles (NFTs) composed of hyperphosphorylated tau are the two major pathological hallmarks of AD [4]. The accumulation of abnormal (argyrophilic) tau starts in the transentorhinal cortex in the earliest stages of AD and spreads through the limbic and association cortices via the.